Blar i forfatter "Bælum, Jacob"

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    • Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses 

      Schostag, Morten; Stibal, Marek; Jacobsen, Carsten S.; Bælum, Jacob; Tas, Neslihan; Elberling, Bo; Jansson, Janet K; Semenchuk, Philipp; Priemé, Anders (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-04-30)
      The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S ...

    • Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples 

      Jacobsen, Carsten Suhr; Nielsen, Tue Kjærgaard; Vester, Jan Kjølhede; Stougaard, Peter; Nielsen, Jeppe Lund; Voriskova, Jana; Winding, Anne; Baldrian, Petr; Liu, Binbin; Frostegård, Åsa; Pedersen, Dorthe; Tveit, Alexander Tøsdal; Svenning, Mette Marianne; Tebbe, Christoph C.; Øvreås, Lise; Jakobsen, Pia Bach; Blazewicz, Steven J.; Hubablek, Valerie; Bertilsson, Stefan; Hansen, Lars Hestbjerg; Cary, S. Craig; Holben, William E.; Ekelund, Flemming; Bælum, Jacob (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-04-09)
      Here we show that a commercial blocking reagent (G2) based on modified eukaryotic DNA significantly improved DNA extraction efficiency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the ...